TOP1MT anticorps (CF®488A)

N° du produit ABIN6154273

L’anticorps Souris Monoclonal anti-TOP1MT a été validé pour IF, FACS et IHC (f). Il convient pour détecter TOP1MT dans des échantillons de Humain.

Aperçu rapide pour TOP1MT anticorps (CF®488A) (ABIN6154273)

Antigène: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TOP1MT est conjugé à/à la CF®488A

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: TOP1MT-488

Détail du produit anti-TOP1MT anticorps (CF®488A)

Fonction: Mouse Monoclonal anti-Topoisomerase-I, MT (TOP1MT/488), CF488A Conjugate

Attributs du produit: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline  pH for optimal activity. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human TOP1MT protein

Isotype: IgG2b kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

A431 cells. Heart, Skeletal muscle, brain or fetal liver.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur TOP1MT

Antigène: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Autre désignation: Topoisomerase-I, MT

Sujet: DNA topoisomerase I, TOP1MT, Topoisomerase I mitochondrial, Type IB Topoisomerase

Poids moléculaire: 70 kDa

ID gène: 116447, 528574

UniProt: Q969P6