POLG anticorps (AA 829-1201)

N° du produit ABIN615477

Cet anticorps anti-POLG Polyclonal Lapin (ABIN615477) détecte spécifiquement POLG dans WB, IHC (p) et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour POLG anticorps (AA 829-1201) (ABIN615477)

Antigène: POLG (Polymerase (DNA Directed), gamma (POLG))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLG est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Détail du produit anti-POLG anticorps

Épitope: AA 829-1201

Specificité: This antibody detects POLG1.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogène: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 829 and 1201 of DNA polymerase gamma.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.7 mg/mL

Buffer: PBS, pH 7, 1 % BSA, 20 % Glycerol and 0.01 % Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C. Dilute only prior to immediate use.

Détails sur POLG

Antigène: POLG (Polymerase (DNA Directed), gamma (POLG))

Autre désignation: POLG1

Sujet: As the only DNA polymerase (pol) present in mitochondria, DNA polymerase gamma (POLG) is necessarily implicated in base excision repair processes to correct oxidative damage to the mitochondrial genome. Therefore, the ability of the catalytic subunit of human POLG has been tested to participate in uracil provoked base excision repair reconstituted in vitro with purified components. Subsequent to actions of uracil DNA glycosylase and apurinic / apyrimidinic endonuclease, human POLG is able to fill a single nucleotide gap in the presence of a 5' terminal deoxyribose phosphate (dRP) flap. The catalytic subunit of human pol gamma catalyzes release of the dRP residue from incised apurinic / apyrimidinic sites to produce a substrate for DNA ligase. Faulty replication of the human mitochondrial genome is thought to be the cause of many diseases. The low selectivity of the mitochondrial DNA polymerase is implicated as the cause of many side effects observed in the treatment of viral infections such as HIV.Synonyms: DNA polymerase subunit gamma-1, MDP1, Mitochondrial DNA polymerase catalytic subunit, POLG, POLGA, PolG-alpha

ID gène: 5428

NCBI Accession: NP_001119603

UniProt: P54098