Topoisomerase II alpha anticorps (AA 1352-1493) (CF®647)

N° du produit ABIN6157527

Cet anticorps Souris Monoclonal détecte spécifiquement Topoisomerase II alpha dans WB, IHC, IF et FACS. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour Topoisomerase II alpha anticorps (AA 1352-1493) (CF®647) (ABIN6157527)

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Topoisomerase II alpha est conjugé à/à la CF®647

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: TOP2A-1361

Détail du produit anti-Topoisomerase II alpha anticorps (CF®647)

Épitope: AA 1352-1493

Fonction: Mouse Monoclonal anti-Topoisomerase-II-alpha (TOP2A/1361), CF647 Conjugate

Attributs du produit: This antibody recognizes a 170 kDa protein, which is identified as topoisomerase IIα. It shows no cross-reaction with topoisomerase IIβ or topoisomerase I. Topo IIα plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIα is also implicated in drug resistance of tumor cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human Topoisomerase II alpha fragment (aa1352-1493) (exact sequence is proprietary)

Isotype: IgG2b kappa

Information d'application

Indications d'application: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

HeLa Cells or Tonsil

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur Topoisomerase II alpha

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Autre désignation: Topoisomerase-II-alpha

Poids moléculaire: 170 kDa

ID gène: 7153, 156346

UniProt: P11388

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases