UBE2F anticorps (N-Term)

N° du produit ABIN615974

L’anticorps anti-UBE2F Polyclonal Chèvre est utilisé pour la détection de UBE2F dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Chien et Porc. Il a été validé pour WB et EIA.

Aperçu rapide pour UBE2F anticorps (N-Term) (ABIN615974)

Antigène: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp UBE2F est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-UBE2F anticorps

Épitope: N-Term

Séquence: SKLKRDDGLK GSRT-C

Specificité: Recogfnizes NEDD8 conjugating enzyme (N Terminus).

Réactivité croisée (Details): Species reactivity (expected):Human, Mouse, Canine (Dog), Pig, Cow.
Species reactivity (tested):Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence from the N Terminus of the protein sequence according to NP_542409.1. Genename: UBE2F

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur UBE2F

Antigène: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Autre désignation: NCE2

Sujet: Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43 % sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.Synonyms: NEDD8-conjugating enzyme 2, UBE2F, Ubiquitin-conjugating enzyme E2 F

ID gène: 140739

NCBI Accession: NP_542409

UniProt: Q969M7