VEGFB anticorps (AA 21-188)

N° du produit ABIN616026

L’anticorps anti-VEGFB Polyclonal Lapin est utilisé pour la détection de VEGFB dans des échantillons de Humain. Il a été validé pour WB.

Aperçu rapide pour VEGFB anticorps (AA 21-188) (ABIN616026)

Antigène: VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VEGFB est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-VEGFB anticorps

Épitope: AA 21-188

Specificité: This antibody detects VEGF-B.

Réactivité croisée (Details): Species reactivity (tested):Human

Purification: Protein-A purified

Immunogène: Highly pure (>95%) recombinant human VEGF-B167 (Ala21-Arg188) derived from E. coli

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antibody is stable at room temperature for up to 1 month. Following reconstitution antibody can be stored at 2-8 °C for up to two weeks or (in aliquots) at -20 °C for longer.

Détails sur VEGFB

Antigène: VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

Autre désignation: VEGF-B

Sujet: Vascular endothelial growth factor B (VEGF-B), also known as vascular endothelial growth factor-related factor (VRF), is a member of the VEGF family of growth factors that share structural and functional similarity (1, 2). Five mammalian members, including VEGF-A, B, C, D and PlGF, have been identified. VEGF family members are disulfide-linked dimeric proteins that are important regulators of physiological and pathological vasculogenesis, angiogenesis and lymphangiogenesis. VEGF-B is expressed in most tissues, especially in heart, skeletal muscle and pancreas. In many tissues, VEGF-B is coexpressed and can heterodimerize with VEGF (3). By alternative splicing, two isoforms of mature VEGF-B containing 167 or 186 amino acid (aa) residues exist (3, 4). The two VEGF-B isoforms have identical amino-terminal cysteine knot VEGF homology domains but the carboxyl end of VEGF-B167 differs from that of VEGF-B186 by the presence of a highly basic cysteine-rich heparin binding domain. Whereas VEGF-B186 is a secreted diffusible protein, VEGF-B167 is sequestered into the cell matrix after secretion. Both VEGF-B isoforms bind VEGF receptor 1 (VEGFR-1), but not VEGFR-2 or VEGFR-3 (5). On endothelial cells, ligation of VEGFR-1 by VEGF-B has been shown to regulate the expression and activity of urokinase type plasminogen activator and plasminogen activator inhibitor 1. VEGF-B167 and a proteolytically processed form of VEGF-B186 (VEGF-B127) also bind neuropilin1 (NP1), a type I transmembrane receptor for semaphorins/collapsins, ligands involved in neuron guidance (6). Besides VEGF-B, NP1 has been shown to bind PlGF-2, VEGF165 and VEGFR-1 (6, 7). The many interactions of NP1 with VEGF ligands and receptor suggest that NP1 may function as a regulator of angiogenesis (7).Synonyms: VEGF-related factor, VEGFB, VRF, Vascular endothelial growth factor B

ID gène: 7423

NCBI Accession: NP_003368

UniProt: P49765

Pathways: Signalisation RTK, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals