Recombinant Chromogranin A anticorps (CF®647)

N° du produit ABIN6160871

Cet anticorps anti-Chromogranin A est un anticorps Lapin Monoclonal détectant Chromogranin A dans IF, IHC et FACS. Adapté pour Humain.

Aperçu rapide pour Recombinant Chromogranin A anticorps (CF®647) (ABIN6160871)

Antigène: Chromogranin A (CHGA)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Chromogranin A est conjugé à/à la CF®647

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: CHGA-1731R

Détail du produit anti-Chromogranin A anticorps (CF®647)

Fonction: Recombinant Rabbit Monoclonal anti-Chromogranin A / CHGA (CHGA/1731R), CF647 Conjugate

Attributs du produit: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human full-length Chromogranin A protein

Isotype: IgG kappa

Information d'application

Indications d'application: Immunohistology (formalin) 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

PC12 cells. Adrenal gland, bowel, parathyroid, pancreas, or pheochromocytoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur Chromogranin A

Antigène: Chromogranin A (CHGA)

Autre désignation: Chromogranin A / CHGA

Sujet: Beta-Granin, CGA, CHGA, Chromogranin A Parathyroid Secretory Protein 1, ER-37, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I, Vasostatin I or II

Poids moléculaire: 68-75 kDa

ID gène: 1113, 150793

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling