Calnexin anticorps (N-Term)

N° du produit ABIN6161470

L’anticorps Souris Monoclonal anti-Calnexin a été validé pour WB, IHC, IF, FACS et ISt. Il convient pour détecter Calnexin dans des échantillons de Humain.

Aperçu rapide pour Calnexin anticorps (N-Term) (ABIN6161470)

Antigène: Calnexin (CANX)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Calnexin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt)

Clone: CANX-1541

Détail du produit anti-Calnexin anticorps

Épitope: N-Term

Fonction: Mouse Monoclonal anti-Calnexin (CANX/1541), Purified, BSA-free

Attributs du produit: This antibody recognizes a protein of 90 kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: Recombinant N-terminal fragment of human Calnexin protein (exact sequence is proprietary)

Isotype: IgG2b kappa

Information d'application

Indications d'application: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

HeLa, MCF-7 or U2OS cells. Kidney or small intestine.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Agent conservateur: Azide free

Détails sur Calnexin

Antigène: Calnexin (CANX)

Autre désignation: Calnexin

Sujet: Calnexin, CANX, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88, P90

Poids moléculaire: 90 kDa

ID gène: 821, 567968

UniProt: P27824

Pathways: Signalisation MAPK, Thyroid Hormone Synthesis