Cyclin B1 anticorps (CF®647)

N° du produit ABIN6161669

Cet anticorps anti-Cyclin B1 est un anticorps Souris Monoclonal détectant Cyclin B1 dans IF, FACS, IP et BP. Adapté pour Humain, Souris et Hamster.

Aperçu rapide pour Cyclin B1 anticorps (CF®647) (ABIN6161669)

Antigène: Cyclin B1 (CCNB1)

Reactivité: Humain, Souris, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin B1 est conjugé à/à la CF®647

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Peptide (BP)

Clone: V92-1

Détail du produit anti-Cyclin B1 anticorps (CF®647)

Fonction: Mouse Monoclonal anti-CyclinB1 (V92.1), CF647 Conjugate

Attributs du produit: This antibody recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Hamster Cyclin B1 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunoprecipitation 1-2 μg/500 μg protein, precipitates active CDK1/cyclin B1 complexes
• Optimal dilution for a specific application should be determined by user

Commentaires:

Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur Cyclin B1

Antigène: Cyclin B1 (CCNB1)

Autre désignation: CyclinB1

Sujet: CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1

Poids moléculaire: 55-62 kDa

ID gène: 891, 23960

UniProt: P14635

Pathways: Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase