Cyclin B1 anticorps (CF®488A)
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- Antigène Voir toutes Cyclin B1 (CCNB1) Anticorps
- Cyclin B1 (CCNB1)
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Reactivité
- Humain, Souris, Hamster
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Cyclin B1 est conjugé à/à la CF®488A
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Peptide (BP)
- Fonction
- Mouse Monoclonal anti-CyclinB1 (V92.1), CF488A Conjugate
- Attributs du produit
- This antibody recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogène
- Hamster Cyclin B1 protein
- Clone
- V92-1
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CCNB1 Anticorps primaire
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- Indications d'application
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Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunoprecipitation 1-2 μg/500 μg protein, precipitates active CDK1/cyclin B1 complexes
- Optimal dilution for a specific application should be determined by user
- Commentaires
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Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Protect from light
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- Antigène
- Cyclin B1 (CCNB1)
- Autre désignation
- CyclinB1 (CCNB1 Produits)
- Synonymes
- anticorps ccnb, anticorps cycb, anticorps cb267, anticorps cycb1, anticorps wu:fa19g04, anticorps wu:fb16d01, anticorps wu:fb16e07, anticorps wu:fi21c01, anticorps ccnb1, anticorps MGC53596, anticorps CCNB, anticorps Ccnb1-rs1, anticorps Ccnb1-rs13, anticorps CycB1, anticorps Cycb-4, anticorps Cycb-5, anticorps Cycb1-rs1, anticorps cyclin B1, anticorps cyclin B1 S homeolog, anticorps ccnb1, anticorps ccnb1.2, anticorps ccnb1.S, anticorps CCNB1, anticorps Ccnb1, anticorps ccnb1.2.S
- Sujet
- CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1
- Poids moléculaire
- 55-62 kDa
- ID gène
- 891, 23960
- UniProt
- P14635
- Pathways
- Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
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