FOXP3 anticorps (CF®488A)

N° du produit ABIN6163127

Cet anticorps anti-FOXP3 est un anticorps Souris Monoclonal détectant FOXP3 dans FACS, IF et IHC (f). Adapté pour Humain, Souris et Singe.

Aperçu rapide pour FOXP3 anticorps (CF®488A) (ABIN6163127)

Antigène: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivité: Humain, Souris, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FOXP3 est conjugé à/à la CF®488A

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: FXP3-197

Détail du produit anti-FOXP3 anticorps (CF®488A)

Fonction: Mouse Monoclonal anti-FOXP3 (FXP3/197), CF488A Conjugate

Attributs du produit: This antibody recognizes a protein of 47-55 kDa, which is identified as FOXP3. Its precise epitope is not known, but it has been mapped to the N-terminal portion of the protein. The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung, with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human full-length FOXP3 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Tonsil or Testis

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur FOXP3

Antigène: FOXP3 (Forkhead Box P3 (FOXP3))

Autre désignation: FOXP3

Poids moléculaire: 47-55 kDa

ID gène: 50943, 247700

UniProt: Q9BZS1

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation