HSPD1 anticorps (CF®568)

N° du produit ABIN6164335

L’anticorps Souris Monoclonal anti-HSPD1 a été validé pour WB, IF, FACS, IHC (f) et ISt. Il convient pour détecter HSPD1 dans des échantillons de Humain, Souris, Rat, Poulet, Singe, Hamster, Chien, Boeuf (Vache), Lapin, Porc, Mouton, Drosophila melanogaster et Xenopus laevis.

Aperçu rapide pour HSPD1 anticorps (CF®568) (ABIN6164335)

Antigène: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reactivité: Humain, Souris, Rat, Poulet, Singe, Hamster, Chien, Boeuf (Vache), Lapin, Porc, Mouton, Drosophila melanogaster, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSPD1 est conjugé à/à la CF®568

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)

Clone: LK1

Détail du produit anti-HSPD1 anticorps (CF®568)

Fonction: Mouse Monoclonal anti-HSP60 (LK1), CF568 Conjugate

Attributs du produit: This antibody recognizes a 60 kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp?s), more appropriately called as stress response proteins (srp?s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone LK1, unlike LK2, This antibody recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human HSP60 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Does not react with bacteria, helminths, or spinach, others not known
• Western blotting 0.25-0.5 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur HSPD1

Antigène: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Autre désignation: HSP60

Poids moléculaire: 60 kDa

ID gène: 3329, 595053

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity