NUMA1 anticorps

N° du produit ABIN6165954

Détail du produit anti-NUMA1 anticorps

Antigène: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NUMA1 est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Fonction: Mouse Monoclonal anti-NuMA (A73-B/D12), Purified, BSA-free

Attributs du produit: This antibody recognizes a phosphorylated protein of 228 kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200 kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: Colon carcinoma 174T cells

Clone: A73-B-D12

Isotype: IgM kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Exponentially growing any cultured human cells. Tonsil or lymph node.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Agent conservateur: Azide free

Détails sur NUMA1

Antigène: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Autre désignation: NuMA (NUMA1 Produits)

Synonymes: anticorps NUMA, anticorps 6720401E04Rik, anticorps AA764025, anticorps AL022610, anticorps AU014979, anticorps NUMA1, anticorps NuMA, anticorps SP-H, anticorps numa, anticorps numa1, anticorps nuclear mitotic apparatus protein 1, anticorps nuclear mitotic apparatus protein 1 S homeolog, anticorps NUMA1, anticorps Numa1, anticorps numa1, anticorps numa1.S

Sujet: Nuclear Mitotic Apparatus Protein 1, NuMA protein, NUMA1, SP-H antigen, Structural nuclear protein,

Poids moléculaire: 228 kDa

ID gène: 4926, 325978

UniProt: Q14980

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase