Nucleolin anticorps

N° du produit ABIN6166144

L’anticorps Souris Monoclonal anti-Nucleolin a été validé pour WB, IHC, IF, FACS et ISt. Il convient pour détecter Nucleolin dans des échantillons de Humain.

Aperçu rapide pour Nucleolin anticorps (ABIN6166144)

Antigène: Nucleolin (NCL)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nucleolin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt)

Clone: NCL-902

Détail du produit anti-Nucleolin anticorps

Fonction: Mouse Monoclonal anti-Nucleolin (NCL/902), Purified, BSA-free

Attributs du produit: This antibody recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: Recombinant human NCL protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Does not react with mouse, rat or cow, others not known

• Immunohistology (formalin)
• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

All human Cells. Testis, Ovary, Uterus, Endometrial or Hodgkin s lymphoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Agent conservateur: Azide free

Détails sur Nucleolin

Antigène: Nucleolin (NCL)

Autre désignation: Nucleolin

Sujet: NCL, Nucl, Nucleolin, Protein C23

Poids moléculaire: 76 kDa

ID gène: 4691, 79110

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization