CD31 anticorps

N° du produit ABIN6169279

L’anticorps Souris Monoclonal anti-CD31 a été validé pour FACS, WB, IF et IHC (f). Il convient pour détecter CD31 dans des échantillons de Humain, Lapin et Cynomolgus.

Aperçu rapide pour CD31 anticorps (ABIN6169279)

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivité: Humain, Lapin, Cynomolgus

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD31 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: C31-7

Détail du produit anti-CD31 anticorps

Fonction: Mouse Monoclonal anti-CD31 / PECAM-1 (C31.7), Purified, with BSA

Attributs du produit: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: Human recombinant CD31 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1.0 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Jurkat cells. Tonsil or Angiosarcoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur CD31

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Autre désignation: CD31 / PECAM-1

Sujet: EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA',

Poids moléculaire: ~100 kDa (endothelium) and ~130 kDa (platelets)

ID gène: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization