Desmin anticorps (CF®568)

N° du produit ABIN6171196

L’anticorps Souris Monoclonal anti-Desmin a été validé pour WB, IHC, IF et FACS. Il convient pour détecter Desmin dans des échantillons de Humain, Rat, Souris, Poulet et Hamster.

Aperçu rapide pour Desmin anticorps (CF®568) (ABIN6171196)

Antigène: Desmin (DES)

Reactivité: Humain, Rat, Souris, Poulet, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Desmin est conjugé à/à la CF®568

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: DES-1711

Détail du produit anti-Desmin anticorps (CF®568)

Fonction: Mouse Monoclonal anti-Desmin (DES/1711), CF568 Conjugate

Attributs du produit: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain ?-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. The antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human full-length desmin protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology (formalin) 1-2 μg/mL

• Immunofluorescence 1-2 μg/mL Flow cytometry 0.5-1 μg/million cells
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

SJRH30 Cells. Uterus or Leiomyosarcoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur Desmin

Antigène: Desmin (DES)

Autre désignation: Desmin

Sujet: CMD1I, CSM1, CSM2, DES, Intermediate filament protein

Poids moléculaire: 52 kDa

ID gène: 1674, 594952

UniProt: P17661