EPO anticorps (AA 28-162)

N° du produit ABIN6174259

L’anticorps Souris Monoclonal anti-EPO a été validé pour IHC, FACS et IF. Il convient pour détecter EPO dans des échantillons de Humain.

Aperçu rapide pour EPO anticorps (AA 28-162) (ABIN6174259)

Antigène: EPO (Erythropoietin (EPO))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EPO est non-conjugé

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: EPO-1367

Détail du produit anti-EPO anticorps

Épitope: AA 28-162

Fonction: Mouse Monoclonal anti-Erythropoietin (EPO/1367), Purified, BSA-free

Attributs du produit: This antibody recognizes a protein of about 37 kDa, which is identified as Erythropoietin (EPO). Erythropoietin is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: Recombinant fragment of human EPO protein (aa28-162) (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application: Immunohistology (formalin) 4-8 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

HepG2 Cells. Heart or Kidney.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Agent conservateur: Azide free

Détails sur EPO

Antigène: EPO (Erythropoietin (EPO))

Autre désignation: Erythropoietin

Classe de substances: Hormone

Sujet: EP, EPO alpha, EPO, Epoetin, Erythropoietin, MVCD2

Poids moléculaire: 37 kDa

ID gène: 2056, 2303

UniProt: P01588

Pathways: Signalistation JAK/STAT, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity