BCL2L1 anticorps (AA 3-14) (CF®647)

N° du produit ABIN6175446

Cet anticorps Souris Monoclonal détecte spécifiquement BCL2L1 dans WB, IF, FACS et IHC (f). Il présente une réactivité envers Humain, Souris, Rat et Porc.

Aperçu rapide pour BCL2L1 anticorps (AA 3-14) (CF®647) (ABIN6175446)

Antigène: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reactivité: Humain, Souris, Rat, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BCL2L1 est conjugé à/à la CF®647

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: BX006 2H12

Détail du produit anti-BCL2L1 anticorps (CF®647)

Épitope: AA 3-14

Fonction: Mouse Monoclonal anti-bcl-x (BX006 + 2H12), CF647 Conjugate

Attributs du produit: This antibody recognizes a protein of 27 kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein (BX006 & 2H12)

Isotype: IgG

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin s lymphoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur BCL2L1

Antigène: BCL2L1 (BCL2-Like 1 (BCL2L1))

Autre désignation: bcl-x

Sujet: Apoptosis regulator Bcl-X, B cell lymphoma 2 like, BCL XL/S, Bcl-2-like protein 1, Bcl2-L-1, BCL2L, Bcl2l1, BCLX, BclXL, BclXs, Protein phosphatase 1 regulatory subunit 52 (PPP1R52)

Poids moléculaire: 27 kDa

ID gène: 598, 516966

UniProt: Q07817

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling