MCM7 anticorps (AA 195-319) (CF®640R)

N° du produit ABIN6177912

Cet anticorps Souris Monoclonal détecte spécifiquement MCM7 dans IHC, IF et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour MCM7 anticorps (AA 195-319) (CF®640R) (ABIN6177912)

Antigène: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MCM7 est conjugé à/à la CF®640R

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: MCM7-1467

Détail du produit anti-MCM7 anticorps (CF®640R)

Épitope: AA 195-319

Fonction: Mouse Monoclonal anti-MCM7 (MCM7/1467), CF640R Conjugate

Attributs du produit: MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human MCM7 protein fragment (aa195-319) (exact sequence is proprietary)

Isotype: IgG2a kappa

Information d'application

Indications d'application: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Raji or Ramos cells. Tonsil

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur MCM7

Antigène: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Autre désignation: MCM7

Sujet: CDC47, DNA replication licensing factor MCM7, MCM7 mini chromosome maintenance deficient 7, Mini chromosome Maintenance 7, Mini chromosome maintenance protein 7, P1.1-MCM3, P1CDC47, P85MCM, PNAS146

Poids moléculaire: 88 kDa

ID gène: 4176, 438720

UniProt: P33993

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA