Cytokeratin 18 anticorps

N° du produit ABIN6179199

Cet anticorps Souris Monoclonal détecte spécifiquement Cytokeratin 18 dans IF, FACS et IHC (f). Il présente une réactivité envers Humain, Souris, Rat, Chien, Porc, Cat, Boeuf (Vache), Hamster, Mouton, Cheval, Furet, Chévre, Marmoset et Singe.

Aperçu rapide pour Cytokeratin 18 anticorps (ABIN6179199)

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivité: Humain, Souris, Rat, Chien, Porc, Cat, Boeuf (Vache), Hamster, Mouton, Cheval, Furet, Chévre, Marmoset, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 18 est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: C-04

Détail du produit anti-Cytokeratin 18 anticorps

Fonction: Mouse Monoclonal anti-Cytokeratin 18 (C-04), Purified, BSA-free

Attributs du produit: This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431

Isotype: IgG1 kappa

Information d'application

Indications d'application: Does not react with chicken or Xenopus, others not known

• Immunohistology formalin-fixed 0.5-1 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

MCF-7, HeLa cells, Breast Cancer

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Agent conservateur: Azide free

Détails sur Cytokeratin 18

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Autre désignation: Cytokeratin 18

Sujet: Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18 Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18

Poids moléculaire: 45 kDa

ID gène: 3875, 406013

UniProt: P05783

Pathways: Apoptose, Caspase Cascade in Apoptosis