beta Catenin anticorps (CF®488A)

N° du produit ABIN6183402

Cet anticorps Souris Monoclonal détecte spécifiquement beta Catenin dans WB, IHC, FACS et IF. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Poulet, Boeuf (Vache) et Chien.

Aperçu rapide pour beta Catenin anticorps (CF®488A) (ABIN6183402)

Antigène: beta Catenin (CATNB) (Catenin, beta (CATNB))

Reactivité: Humain, Souris, Rat, Poulet, Boeuf (Vache), Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp beta Catenin est conjugé à/à la CF®488A

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 15B8

Détail du produit anti-beta Catenin anticorps (CF®488A)

Fonction: Mouse Monoclonal anti-Catenin, beta (15B8), CF488A Conjugate

Attributs du produit: Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant chicken ?-catenin

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tis, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

HeLa or MCF-7 cells. Breast carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur beta Catenin

Antigène: beta Catenin (CATNB) (Catenin, beta (CATNB))

Autre désignation: Catenin, beta

Sujet: Cadherin associated protein), beta 1 88  kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1

Poids moléculaire: 92 kDa

ID gène: 1499, 476018

UniProt: P35222

Pathways: Peptide Hormone Metabolism