beta Catenin anticorps (CF®594)

N° du produit ABIN6183670

L’anticorps Lapin Polyclonal anti-beta Catenin a été validé pour WB, FACS, IF et IHC (f). Il convient pour détecter beta Catenin dans des échantillons de Humain et Souris.

Aperçu rapide pour beta Catenin anticorps (CF®594) (ABIN6183670)

Antigène: beta Catenin (CATNB) (Catenin, beta (CATNB))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp beta Catenin est conjugé à/à la CF®594

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: PAb

Détail du produit anti-beta Catenin anticorps (CF®594)

Fonction: Rabbit Polyclonal anti-Beta-Catenin (p120), CF594 Conjugate

Attributs du produit: Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: A synthetic peptide from the middle of beta-Catenin (p120) protein

Isotype: IgG kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

HeLa or MCF-7 cells. Breast carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur beta Catenin

Antigène: beta Catenin (CATNB) (Catenin, beta (CATNB))

Autre désignation: Beta-Catenin

Sujet: Cadherin associated protein), beta 1 88  kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1

Poids moléculaire: 92 kDa

ID gène: 1499, 476018

UniProt: P35222

Pathways: Peptide Hormone Metabolism