CD40 anticorps (CF®543)

N° du produit ABIN6184906

L’anticorps Souris Monoclonal anti-CD40 a été validé pour FACS, WB, IHC et IF. Il convient pour détecter CD40 dans des échantillons de Humain.

Aperçu rapide pour CD40 anticorps (CF®543) (ABIN6184906)

Antigène: CD40

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD40 est conjugé à/à la CF®543

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: C40-1605

Détail du produit anti-CD40 anticorps (CF®543)

Fonction: Mouse Monoclonal anti-CD40 / TNFRSF5 (C40/1605), CF543 Conjugate

Attributs du produit: CD40 is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is expressed on B-lymphocytes, follicular dendritic cells, bone marrow-derived dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zones of secondary lymphoid organs. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human full-length CD40 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 1-2 μg/mL Flow cytometry 0.5-1 μg/million cells
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

L-428 Cells. Tonsil, Spleen or Thymus

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur CD40

Antigène: CD40

Autre désignation: CD40 / TNFRSF5

Sujet: B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, GP39, HIGM1, IGM, IMD3, p50, TBAM, TNF receptor superfamily member 5, TNFRSF5, TRAP

Poids moléculaire: 43 kDa

ID gène: 958, 472860

UniProt: P25942

Pathways: Signalisation NF-kappaB, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints