Transferrin Receptor anticorps (AA 94-212) (CF®488A)

N° du produit ABIN6185340

Cet anticorps Souris Monoclonal détecte spécifiquement Transferrin Receptor dans FACS, WB, IHC, ELISA, IF et Coat. Il présente une réactivité envers Humain.

Aperçu rapide pour Transferrin Receptor anticorps (AA 94-212) (CF®488A) (ABIN6185340)

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Transferrin Receptor est conjugé à/à la CF®488A

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Coating (Coat)

Clone: TFRC-1817

Détail du produit anti-Transferrin Receptor anticorps (CF®488A)

Épitope: AA 94-212

Fonction: Mouse Monoclonal anti-CD71 / Transferrin Receptor (TFRC/1817), CF488A Conjugate

Attributs du produit: This antibody recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human TFRC protein fragment (aa94-212) (exact sequence is proprietary)

Isotype: IgG2b kappa

Information d'application

Indications d'application: Flow cytometry 0.5-1 μg/million cells in 0.1 mL

• Immunohistology (formalin) 1-2 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• ELISA 2-4 μg/mL for coating (order Ab without BSA)
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

PC3 cells. Human placenta or breast carcinoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur Transferrin Receptor

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Autre désignation: CD71 / Transferrin Receptor

Sujet: Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR

Poids moléculaire: 85-95 kDa (monomer), 190 kDa (dimer)

ID gène: 7037, 529618

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis