CD34 anticorps

N° du produit ABIN6185811

Cet anticorps Souris Monoclonal détecte spécifiquement CD34 dans FACS, IF, IHC (f) et ISt. Il présente une réactivité envers Humain.

Aperçu rapide pour CD34 anticorps (ABIN6185811)

Antigène: CD34

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD34 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)

Clone: HPCA1-763

Détail du produit anti-CD34 anticorps

Fonction: Mouse Monoclonal anti-CD34 (HPCA1/763), Purified, BSA-free

Attributs du produit: This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels > 85 % of angiosarcoma and Kaposi s sarcoma, but with a lower specificity. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: Recombinant full-length human HPCA1 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

KG-1 cells, Tonsil, or Angiosarcoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Agent conservateur: Azide free

Détails sur CD34

Antigène: CD34

Autre désignation: CD34

Sujet: Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Poids moléculaire: 90-110 kDa

ID gène: 947, 374990

UniProt: P28906