Neurofilament anticorps (Biotin)

N° du produit ABIN6188767

L’anticorps Souris Monoclonal anti-Neurofilament a été validé pour FACS, IHC (f) et IF. Il convient pour détecter Neurofilament dans des échantillons de Humain, Rat, Poulet, Porc et Souris.

Aperçu rapide pour Neurofilament anticorps (Biotin) (ABIN6188767)

Antigène: Neurofilament

Reactivité: Humain, Rat, Poulet, Porc, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Neurofilament est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF)

Clone: NF421 NFL-736

Détail du produit anti-Neurofilament anticorps (Biotin)

Fonction: Mouse Monoclonal anti-Neurofilament (H+L) (NF421 + NFL736), Biotin Conjugate

Attributs du produit: This MAb reacts with a 200 kDa and 68 kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human neurofilament heavy sub-unit (RT-97) & light sub-unit (NR-4)

Isotype: IgG

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1:25-1:50
• Optimal dilution for a specific application should be determined by user

Commentaires:

Brain, Neuroblastoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur Neurofilament

Antigène: Neurofilament

Poids moléculaire: 200 kDa & 68 kDa

ID gène: 4744, 198760

UniProt: P12036