Elastase 3B anticorps (AA 82-238) (CF®640R)
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- Antigène Voir toutes Elastase 3B (ELA3B) Anticorps
- Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))
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Épitope
- AA 82-238
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Reactivité
- Humain, Souris, Rat
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Elastase 3B est conjugé à/à la CF®640R
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Application
- ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat), Immunohistochemistry (IHC)
- Fonction
- Mouse Monoclonal anti-CELA3B / ELA3B (CELA3B/1257), CF640R Conjugate
- Attributs du produit
- This MAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogène
- Recombinant human CELA3B protein fragment (aa82-238) (exact sequence is proprietary)
- Clone
- CELA3B-1257
- Isotype
- IgG1
- Top Product
- Discover our top product ELA3B Anticorps primaire
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- Indications d'application
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- ELISA 2-4 μg/mL for coating (order Ab without BSA)
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Commentaires
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Panc-28 or PANC1 cells. Pancreas
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Protect from light
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- Antigène
- Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))
- Autre désignation
- CELA3B / ELA3B (ELA3B Produits)
- Synonymes
- anticorps CBPP, anticorps E1, anticorps EL-1, anticorps ELA3B, anticorps 0910001F22Rik, anticorps 2310074F01Rik, anticorps AI504000, anticorps Ela3, anticorps Ela3b, anticorps ela3b, anticorps MGC82867, anticorps ELA1, anticorps Cela3b, anticorps CELA3B, anticorps chymotrypsin like elastase family member 3B, anticorps chymotrypsin-like elastase family, member 3B, anticorps elastase 3B, pancreatic, anticorps proproteinase E, anticorps chymotrypsin like elastase family member 3B L homeolog, anticorps chymotrypsin-like elastase family member 3B, anticorps CELA3B, anticorps Cela3b, anticorps ela3b, anticorps LOC478196, anticorps cela3b.L, anticorps LOC100717936, anticorps LOC100071692
- Sujet
- Chymotrypsin like elastase family member 3B (CELA3B), ELA3B, Elastase IIIB, Protease E
- Poids moléculaire
- 17 kDa
- ID gène
- 23436, 728752
- UniProt
- P08861
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