Elastase 3B anticorps (AA 82-238) (Biotin)

N° du produit ABIN6191541

L’anticorps Souris Monoclonal anti-Elastase 3B a été validé pour WB, IHC, ELISA, Coat, IF et FACS. Il convient pour détecter Elastase 3B dans des échantillons de Humain.

Aperçu rapide pour Elastase 3B anticorps (AA 82-238) (Biotin) (ABIN6191541)

Antigène: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Elastase 3B est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Coating (Coat), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: CELA3B-1757

Détail du produit anti-Elastase 3B anticorps (Biotin)

Épitope: AA 82-238

Fonction: Mouse Monoclonal anti-CELA3B / ELA3B (CELA3B/1757), Biotin Conjugate

Attributs du produit: This MAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human CELA3B protein fragment (aa82-238) (exact sequence is proprietary)

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• ELISA 2-4 μg/mL for coating (order Ab without BSA)
• Immunofluorescence 0.5-1 μg/mL Western blotting 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Panc-28 or PANC1 cells. Pancreas

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur Elastase 3B

Antigène: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Autre désignation: CELA3B / ELA3B

Sujet: Chymotrypsin like elastase family member 3B (CELA3B), ELA3B, Elastase IIIB, Protease E

Poids moléculaire: 17 kDa

ID gène: 23436, 728752

UniProt: P08861