DMPO anticorps

N° du produit ABIN619793

L’anticorps anti-DMPO Monoclonal Souris est utilisé pour la détection de DMPO. Il a été validé pour IP, ELISA, WB, ICC et IST.

Aperçu rapide pour DMPO anticorps (ABIN619793)

Antigène: DMPO

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunoprecipitation (IP), ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immuno-Spin Trapping (IST)

Clone: N1664A

Détail du produit

Fonction: Anti-DMPO | DMPO nitrone adduct (clone N1664A)

Réactivité croisée (Details): DMPO (species independent)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: DMPO (species independent)

Purification: Protein G purified

Immunogène: 5

Isotype: IgG1

Information d'application

Indications d'application: 1-10 μg/mL (ELISA), 10 μg/mL (ICC), 25 μg (IP), 1-10 μg/mL (WB)

Commentaires:

Important note for immunoprecipitation: do not link antibody to matrix prior to use as this causes large loss of reactivity to DMPO adduct.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Total IgG. Protein G purified.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store at -20°C for one year, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Date de péremption: 12 months

Détail du antigène

Antigène: DMPO

Classe de substances: Chemical

Sujet:

Background: DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.

Additional information: Antibody is purified on Protein G and present in TBS pH 7.4 with 0.05 % sodium azide as preservative and 50 % glycerol.DMPO antibody in dilution of 1: 1000 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colormetric immunoblot analysis using goat anti-mouse IgG HRP conjugated secondary antibody.

Poids moléculaire: 90 kDa