DMPO anticorps

N° du produit ABIN6655016

Détail du produit

Antigène: DMPO

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunoprecipitation (IP), ELISA, Fluorescence Microscopy (FM)

Réactivité croisée: Toutes les espèces

Purification: Anti-DMPO Antibody was purified by Protein G chromatography. DMPO is species independant. This antibody recognzies DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA. Cross-reactivity with DMPO from other sources has not been determined. Neuroscience research.

Immunogène:

Immunogen: DMPO Antibody was produced in mice by repeated immunizations with 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.

Immunogen Type: Other

Clone: N1664A

Isotype: IgG1

Information d'application

Indications d'application:

Application Note: Anti-DMPO Antibody is suitable for use in Immuno-spin trapping, ELISA, WB, IF microscopy and IP. Expect a band approximately ~90 kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: 25 μg

ELISA Dilution: 1-10 μg/mL

IF Microscopy Dilution: 10 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Tris Cl, 0.15 M Sodium Chloride, 0.001 M EDTA, pH 7.4

Stabilizer: 50 % (v/v) Glycerol

0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Détail du antigène

Antigène: DMPO

Classe de substances: Chemical

Sujet:

Synonyms: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N oxide

Background: The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems. Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals.