RPA1 anticorps

N° du produit ABIN6241400

Cet anticorps Souris Monoclonal détecte spécifiquement RPA1 dans WB, IP et ICC. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour RPA1 anticorps (ABIN6241400)

Antigène: RPA1 (Replication Protein A1, 70kDa (RPA1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RPA1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Détail du produit anti-RPA1 anticorps

Immunogène: Recombinant Protein

Information d'application

Indications d'application: IP: 1:500. WB: 1:1000. ICC: 1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C

Détails sur RPA1

Antigène: RPA1 (Replication Protein A1, 70kDa (RPA1))

Autre désignation: RPA70

Sujet: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:< a href="http://www.uniprot.org/citations/9430682" target="_blank">9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:< a href="http://www.uniprot.org/citations/24332808" target="_blank">24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:< a href="http://www.uniprot.org/citations/17765923" target="_blank">17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:< a href="http://www.uniprot.org/citations/7697716" target="_blank">7697716). Plays also a role in base excision repair (BER) probably through interaction with UNG (PubMed:< a href="http://www.uniprot.org/citations/9765279" target="_blank">9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance (PubMed:< a href="http://www.uniprot.org/citations/17959650" target="_blank">17959650). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2- containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:< a href="http://www.uniprot.org/citations/19996105" target="_blank">19996105).

UniProt: P27694

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA