SUMO2/3 anticorps

N° du produit ABIN6251816

Cet anticorps anti-SUMO2/3 Monoclonal Souris (Clone SPM572) (ABIN6251816) détecte spécifiquement SUMO2/3 dans WB, IF, FACS et IHC. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour SUMO2/3 anticorps (ABIN6251816)

Antigène: SUMO2/3 (Small Ubiquitin Related Modifier 2/3 (SUMO2/3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SUMO2/3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: SPM572

Détail du produit anti-SUMO2/3 anticorps

Purification: Protein G affinity chromatography

Immunogène: Recombinant human protein was used as the immunogen for this SUMO-2/3 antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Western blot: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the SUMO-2/3 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur SUMO2/3

Antigène: SUMO2/3 (Small Ubiquitin Related Modifier 2/3 (SUMO2/3))

Autre désignation: SUMO-2/3

Sujet: This mAb reacts with both SUMO-2 and SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, 2 and 3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include MDM2, p53, PML and RanGap1. SUMO-2 and 3 contribute to a greater percentage of protein modification than does SUMO-1 and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer s disease.

ID gène: 6613

UniProt: P61956