S100A8/A9 Complex (Calprotectin) anticorps

N° du produit ABIN6251842

L’anticorps Souris Monoclonal anti-S100A8/A9 Complex (Calprotectin) a été validé pour FACS, IF et IHC (p). Il convient pour détecter S100A8/A9 Complex (Calprotectin) dans des échantillons de Humain.

Aperçu rapide pour S100A8/A9 Complex (Calprotectin) anticorps (ABIN6251842)

Antigène: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp S100A8/A9 Complex (Calprotectin) est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CAGB-426

Détail du produit anti-S100A8/A9 Complex (Calprotectin) anticorps

Purification: PEG precipitation

Immunogène: Recombinant human protein was used as the immunogen for the Calgranulin A + B antibody.

Isotype: IgM kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (Formalin-fixed. Not suitable for frozen tissues.): 0.5-1 μg/mL for 30 min at RT (1)

Optimal dilution of the Calgranulin A + B antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur S100A8/A9 Complex (Calprotectin)

Antigène: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Autre désignation: Calgranulin A + B

Sujet: Recognizes a 12-14  kDa doublet of Calgranulin A/B (also known as S100A8/A9 or MRP-8/14), expressed by granulocytes, monocytes and by tissue macrophages. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This mAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

ID gène: 6280

UniProt: P06702

Pathways: S100 Proteins