dsDNA anticorps

N° du produit ABIN6252090

Cet anticorps Souris Monoclonal détecte spécifiquement dsDNA dans FACS, IF, IHC (p) et ICC. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour dsDNA anticorps (ABIN6252090)

Antigène: dsDNA (Double-Stranded DNA (dsDNA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp dsDNA est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: SPM603

Détail du produit anti-dsDNA anticorps

Purification: Protein G affinity chromatography

Immunogène: Nuclei of Burkitt's cells were used as the immunogen for the Double Stranded DNA antibody.

Isotype: IgG3 kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Immunocytochemistry (Acetone-fixed): 0.5-1 μg/mL for 30 min at RT

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)

Optimal dilution of the Double Stranded DNA antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur dsDNA

Antigène: dsDNA (Double-Stranded DNA (dsDNA))

Autre désignation: Double Stranded DNA

Classe de substances: Nucleotide

Sujet: Marker antibodies are useful in identification of organelles in cells, tissues, and biochemical preparations. This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.