Nucleolin anticorps

N° du produit ABIN6252098

Cet anticorps anti-Nucleolin est un anticorps Souris Monoclonal détectant Nucleolin dans WB, IF, FACS et IHC (p). Adapté pour Humain et Souris.

Aperçu rapide pour Nucleolin anticorps (ABIN6252098)

Antigène: Nucleolin (NCL)

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nucleolin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NPC23-2

Détail du produit anti-Nucleolin anticorps

Purification: Protein G affinity chromatography

Immunogène: Recombinant human protein was used as the immunogen for the Nucleolin antibody.

Isotype: IgG1

Information d'application

Indications d'application: IF: 1-2 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Western blot: 1-2 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Titering of the Nucleolin antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur Nucleolin

Antigène: Nucleolin (NCL)

Autre désignation: Nucleolin

Sujet: Nucleolin (NCL) is the major nucleolar phosphoprotein of growing eukaryotic cells. It is located mainly in dense fibrillar regions of the nucleolus. NCL is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

ID gène: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization