TOX3 anticorps (AA 200-400)

N° du produit ABIN6252144

Cet anticorps Souris Monoclonal détecte spécifiquement TOX3 dans FACS, WB, IF et IHC (p). Il présente une réactivité envers Humain.

Aperçu rapide pour TOX3 anticorps (AA 200-400) (ABIN6252144)

Antigène: TOX3 (TOX High Mobility Group Box Family Member 3 (TOX3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TOX3 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TOX3-1123

Détail du produit anti-TOX3 anticorps

Épitope: AA 200-400

Purification: Protein G affinity chromatography

Immunogène: A recombinant fragment (139 amino acid residues around aa 200-400) from the human protein was used as the immunogen for the TOX3 antibody.

Isotype: IgG2b kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the TOX3 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0 or 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur TOX3

Antigène: TOX3 (TOX High Mobility Group Box Family Member 3 (TOX3))

Autre désignation: TOX3

Sujet: It recognizes a 63  kDa protein, which is identified as TOX3. It contains a high mobility group (HMG)-box, which regulates Ca2+-dependent transcription in neurons through interaction with the cAMP-response-element-binding protein (CREB). TOX3 appears to be associated with breast cancer susceptibility and is expressed downstream of a cytoprotective cascade together with CITED1, a transcriptional regulator that does not bind directly to DNA. TOX3 is predominantly expressed in the brain and forms homodimers. TOX3 overexpression protects neuronal cells from cell death caused by endoplasmic reticulum stress or BAX overexpression through the induction of anti-apoptotic transcripts and repression of pro-apoptotic transcripts.

ID gène: 27324

UniProt: O15405

Pathways: Chromatin Binding