CD16 anticorps

N° du produit ABIN6252332

Cet anticorps anti-CD16 est un anticorps Souris Monoclonal détectant CD16 dans FACS, IF et Func. Adapté pour Humain.

Aperçu rapide pour CD16 anticorps (ABIN6252332)

Antigène: CD16

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD16 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: HO-80

Détail du produit anti-CD16 anticorps

Purification: Protein G affinity chromatography

Immunogène: PBL s from a NK-leukemia patient were used as the immunogen for the CD16 antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Functional Activity (Order SAF formulation)

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Optimal dilution of the CD16 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur CD16

Antigène: CD16

Autre désignation: CD16

Sujet: It recognizes CD16 (Fcgamma,RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80  kDa. Two similar genes represent CD16, CD16A (Fcgamma,RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fcgamma,RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

ID gène: 2214

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process