CD45RO anticorps

N° du produit ABIN6252434

Cet anticorps anti-CD45RO Monoclonal Souris (Clone SPM125) (ABIN6252434) détecte spécifiquement CD45RO dans FACS, IF et IHC. L’anticorps est réactif avec des échantillons de Humain, Chimpanzé et Marmoset.

Aperçu rapide pour CD45RO anticorps (ABIN6252434)

Antigène: CD45RO

Reactivité: Humain, Chimpanzé, Marmoset

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD45RO est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: SPM125

Détail du produit anti-CD45RO anticorps

Purification: Protein G affinity chromatography

Immunogène: Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as immunogen for this anti-CD45RO antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells

Immunofluorescence: 0.5-1 μg/mL

Immunohistology (FFPE): 0.25-0.5 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Due to variation in protocol and secondary antibody used, the anti-CD45RO antibody may need to be titered for optimal performance.

1. FFPE staining requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur CD45RO

Antigène: CD45RO

Autre désignation: CD45RO

Sujet: Recognizes a 180-185  kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

ID gène: 5788

UniProt: P08575