MCM7 anticorps (AA 195-319)

N° du produit ABIN6252450

Cet anticorps Souris Monoclonal détecte spécifiquement MCM7 dans WB, IF, FACS et IHC (p). Il présente une réactivité envers Humain.

Aperçu rapide pour MCM7 anticorps (AA 195-319) (ABIN6252450)

Antigène: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MCM7 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MCM7-1466

Détail du produit anti-MCM7 anticorps

Épitope: AA 195-319

Purification: Protein G affinity chromatography

Immunogène: Amino acids 195-319 were used as the immunogen for the MCM7 antibody.

Isotype: IgG2b

Information d'application

Indications d'application: IF: 0.5-1 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

WB: 0.5-1 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 min at RT

The stated application concentrations are suggested starting amounts. Titration of the MCM7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur MCM7

Antigène: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Autre désignation: MCM7

Sujet: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. [UniProt]

ID gène: 4176

UniProt: P33993

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA