MCM7 anticorps (AA 195-319)

N° du produit ABIN6252451

L’anticorps Souris Monoclonal anti-MCM7 a été validé pour WB, IF, FACS et IHC (p). Il convient pour détecter MCM7 dans des échantillons de Humain.

Aperçu rapide pour MCM7 anticorps (AA 195-319) (ABIN6252451)

Antigène: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MCM7 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MCM7-1467

Détail du produit anti-MCM7 anticorps

Épitope: AA 195-319

Purification: Protein G affinity chromatography

Immunogène: A human partial recombinant protein corresponding to amino acids 195-319 was used as the immunogen for the MCM7 antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Immunofluorescence: 0.5-1 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Titering of the MCM7 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur MCM7

Antigène: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Autre désignation: MCM7

Sujet: MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.

ID gène: 4176

UniProt: P33993

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA