GJB1 anticorps (Cytoplasmic Loop)

N° du produit ABIN6252464

Cet anticorps anti-GJB1 est un anticorps Souris Monoclonal détectant GJB1 dans WB, IF, FACS et IHC (fro). Adapté pour Humain, Rat et Souris.

Aperçu rapide pour GJB1 anticorps (Cytoplasmic Loop) (ABIN6252464)

Antigène: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GJB1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: M12-13

Détail du produit anti-GJB1 anticorps

Épitope: Cytoplasmic Loop

Purification: Protein G affinity chromatography

Immunogène: Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1).

Isotype: IgG

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

WB: 0.5-1 μg/mL

IHC (Frozen): 0.5-1 μg/mL for 30 min at RT

Optimal dilution of the Connexin 32 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur GJB1

Antigène: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Autre désignation: Connexin 32

Sujet: This Ab recognizes a protein of 27-32  kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.

ID gène: 2705

UniProt: P08034

Pathways: Cell-Cell Junction Organization