GJB1 anticorps

N° du produit ABIN6252465

Cet anticorps Rat Monoclonal détecte spécifiquement GJB1 dans WB, IF, FACS et IHC (fro). Il présente une réactivité envers Rat et Souris.

Aperçu rapide pour GJB1 anticorps (ABIN6252465)

Antigène: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivité: Rat, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp GJB1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: R5-21C

Détail du produit anti-GJB1 anticorps

Purification: Protein G affinity chromatography

Immunogène: Mouse liver DOC-JR-plasma membranes were used as the immunogen for the Connexin 32 antibody.

Isotype: IgG2a

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

WB: 0.5-1 μg/mL

IHC (Frozen): 1-2 μg/mL for 30 min at RT

Optimal dilution of the Connexin 32 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur GJB1

Antigène: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Autre désignation: Connexin 32

Sujet: This Ab recognizes a protein of 27-32  kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.

ID gène: 14618

UniProt: P28230

Pathways: Cell-Cell Junction Organization