FBXO5 anticorps (N-Term)

N° du produit ABIN6252698

Cet anticorps anti-FBXO5 est un anticorps Souris Monoclonal détectant FBXO5 dans WB, IF et IHC (p). Adapté pour Humain.

Aperçu rapide pour FBXO5 anticorps (N-Term) (ABIN6252698)

Antigène: FBXO5 (F-Box Protein 5 (FBXO5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FBXO5 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: FBXP5

Détail du produit anti-FBXO5 anticorps

Épitope: N-Term

Purification: Protein G affinity chromatography

Immunogène: A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Western blot: 1-2 μg/mL

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the EMI-1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur FBXO5

Antigène: FBXO5 (F-Box Protein 5 (FBXO5))

Autre désignation: EMI-1

Sujet: Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI-1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI-1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

ID gène: 26271

UniProt: Q9UKT4

Pathways: Mitotic G1-G1/S Phases