Topoisomerase II alpha anticorps (AA 1352-1493)

N° du produit ABIN6252772

L’anticorps Souris Monoclonal anti-Topoisomerase II alpha a été validé pour WB, IHC, IF et FACS. Il convient pour détecter Topoisomerase II alpha dans des échantillons de Humain et Souris.

Aperçu rapide pour Topoisomerase II alpha anticorps (AA 1352-1493) (ABIN6252772)

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Topoisomerase II alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: TOP2A-1361

Détail du produit anti-Topoisomerase II alpha anticorps

Épitope: AA 1352-1493

Purification: Protein G affinity chromatography

Immunogène: Amino acids 1352-1493 from the human protein were used as the immunogen for this Topoisomerase II alpha antibody.

Isotype: IgG2b kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml

IF: 1-2 μg/mL

Western blot: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur Topoisomerase II alpha

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Autre désignation: TOP2A

Sujet: This mAb recognizes an ~170  kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

ID gène: 7153

UniProt: P11388

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases