Tubulin anticorps

N° du produit ABIN6253484

Détail du produit anti-Tubulin anticorps

Antigène: Tubulin (TUB)

Reactivité: Humain, Souris, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Tubulin est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Fonction: anti-Tubulin (glycylated), pAb (Gly-pep1)

Attributs du produit: Polyclonal Antibody. This antibody recognizes mono or bi-glycylated Tubulins. The activity of glycylating enzymes (TTLL3 and TTLL8) in cultured cells leads mainly to the modification of alpha- and beta-tubulin, but also of other, yet unidentified protein substrates also detected by the antibody Gly-pep1. In immunofluorescence labeling, the antibody strongly labels glycylated microtubules. Source: Rabbit. Applications: ICC, IP, WB. Liquid. In PBS containing 0.02 % sodium azide. Microtubules are key cytoskeletal elements that are found in all eukaryotic cells. Microtubules fulfil a large range of different functions, which are thought to be controlled by the 'tubulin code' - mechanism to generate distinct microtubule identities. One mechanism to label specific microtubules are tubulin posttranslational modifications (PTMs), of which a large variety exists. One of these modifications is glycylation, which is generated by the addition of secondary (branched) glycine chains to the main (primary) peptide chain of the protein. The length of these branch chains can vary from one to more than 20 glycine residues. Glycylation is catalysed by the enzymes TTLL3, TTLL8 and TTLL10 from the tubulin tyrosine ligase-like (TTLL) family. Especially TTLL3 and TTLL8 are essential for the initiation of the glycylation because the generate the nascent glycine chain. The Gly-pep1 antibody was raised against a peptide mimicking beta2-tubulin (TUBB2A) with a single glycine branch on E437. The antibody specifically detects glycylated tubulin, and also other yet unknown glycylation substrates in cells as well as in tissues. As glycyation of microtubules is particularly found in cilia and flagella, Gly-pep1 labels motile cilia as well as primary cilia.

Pureté: >95 % (SDS-PAGE)

Immunogène: Synthetic peptide corresponding to D431EQGEFE(G-COOH*)EEEG441-NH2 of human Tubulin beta-2A chain (*secondary glycine branched from gamma-carboxyl group of glutamate as isopeptide bond).

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. In PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

Détails sur Tubulin

Antigène: Tubulin (TUB)

Autre désignation: Tubulin (TUB Produits)

Synonymes: anticorps 4t, anticorps B4t, anticorps BETA 98B, anticorps CG4869, anticorps DTB1, anticorps Dmbeta4, anticorps Dmel\\CG4869, anticorps T, anticorps Tub, anticorps beta-Tub, anticorps beta-Tub97EF, anticorps beta-tub, anticorps beta4, anticorps beta4t, anticorps beta97F, anticorps betaTub4, anticorps betaTub98BC, anticorps betaTub98C, anticorps beta-Tubulin at 97EF, anticorps betaTub97EF

Sujet:

Product Description: Microtubules are key cytoskeletal elements that are found in all eukaryotic cells. Microtubules fulfil a large range of different functions, which are thought to be controlled by the 'tubulin code' - mechanism to generate distinct microtubule identities. One mechanism to label specific microtubules are tubulin posttranslational modifications (PTMs), of which a large variety exists. One of these modifications is glycylation, which is generated by the addition of secondary (branched) glycine chains to the main (primary) peptide chain of the protein. The length of these branch chains can vary from one to more than 20 glycine residues. Glycylation is catalysed by the enzymes TTLL3, TTLL8 and TTLL10 from the tubulin tyrosine ligase-like (TTLL) family. Especially TTLL3 and TTLL8 are essential for the initiation of the glycylation because the generate the nascent glycine chain. The Gly-pep1 antibody was raised against a peptide mimicking beta2-tubulin (TUBB2A) with a single glycine branch on E437. The antibody specifically detects glycylated tubulin, and also other yet unknown glycylation substrates in cells as well as in tissues. As glycyation of microtubules is particularly found in cilia and flagella, Gly-pep1 labels motile cilia as well as primary cilia (Gadadhar et al. 2017)