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APEX1 anticorps

Cet anticorps anti-APEX1 est un anticorps Lapin Polyclonal détectant APEX1 dans WB. Adapté pour Arabidopsis thaliana et Spinach. Ce Primary Antibody a été cité dans 4+ publications.
N° du produit ABIN6254847

Aperçu rapide pour APEX1 anticorps (ABIN6254847)

Antigène

Voir toutes APEX1 Anticorps
APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivité

  • 94
  • 46
  • 42
  • 8
  • 7
  • 7
  • 7
  • 6
  • 6
  • 6
  • 5
  • 4
  • 2
  • 1
  • 1
Arabidopsis thaliana, Spinach

Hôte

  • 77
  • 18
  • 2
Lapin

Clonalité

  • 77
  • 20
Polyclonal

Conjugué

  • 67
  • 4
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp APEX1 est non-conjugé

Application

  • 84
  • 32
  • 29
  • 25
  • 22
  • 15
  • 8
  • 7
  • 7
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Attributs du produit

    Expected / apparent Molecular Weight of the Antigene: 25-38 kDa for A. thaliana
    Antibody detection limit is 7 pg, 20 fmol, linear range of logit/logplot is 0.023-15pmol,midrange (B/Bo=50%) is 120 pg, 342 fmol .

    Purification

    serum

    Immunogène

    synthetic peptide derived from Arabidopsis thaliana tAPX (acession At1g77490) and sAPX (At1g08390) protein sequences, coupled to BSA. Five out of twelve amino acids are also identical with cAPX1 (At1g07890), cPX2 (At1g09640) and pAPX (At1g35000)
  • Indications d'application

    Recommended Dilution: 1 : 2000 with standard ECL (WB).

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    For reconstitution add 200 µL of sterile water

    Conseil sur la manipulation

    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

    Stock

    -20 °C
  • Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." dans: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).

    Lehtima: "Nodularin uptake and induction of oxidative stress in spinach (Spinachia oleracea)." dans: Journal of plant physiology, (2010) (PubMed).

    Hideg, Kuos, Schreiber: "Imaging of NPQ and ROS formation in tobacco leaves: heat inactivation of the water-water cycle prevents down-regulation of PSII." dans: Plant & cell physiology, Vol. 49, Issue 12, pp. 1879-86, (2008) (PubMed).

    Kangasjaervi, Lepistoe, Haennikaeinen, Piippo, Luomala, Aro, Rintamaeki: "Diverse roles for chloroplast stromal and thylakoid-bound ascorbate peroxidases in plant stress responses." dans: The Biochemical journal, Vol. 412, Issue 2, pp. 275-85, (2008) (PubMed).

  • Antigène

    APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

    Autre désignation

    APX

    Sujet

    AGI Code: At1g77490
    APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water. Cellular localization includes chloroplast (tAPX and sAPX), cytosol (cAPX) and peroxisome (pAPX).

    Poids moléculaire

    25-38 kDa for A. thaliana

    UniProt

    Q42564, Q05431, Q1PER6, Q42592, Q42593

    Pathways

    Réparation de l'ADN, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
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