PKM anticorps (pSer37)

N° du produit ABIN6255266

Cet anticorps Lapin Polyclonal détecte spécifiquement PKM dans ELISA, WB et IHC. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour PKM anticorps (pSer37) (ABIN6255266)

Antigène: PKM (Pyruvate Kinase M1/2 (PKM))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKM est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-PKM anticorps

Épitope: pSer37

Specificité: Phospho-PKM2 (Ser37) Antibody detects endogenous levels of PKM2 only when phosphorylated at Ser37.

Homologie: Pig,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogène: A synthesized peptide derived from human PKM2 around the phosphorylation site of Ser37.

Isotype: IgG

Information d'application

Indications d'application: WB 1:1000-3000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur PKM

Antigène: PKM (Pyruvate Kinase M1/2 (PKM))

Autre désignation: PKM

Sujet:

Description: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Gene: PKM

Poids moléculaire: 58 kDa

ID gène: 5315

UniProt: P14618

Pathways: L'effet Warburg