EIF3B anticorps (pSer154)

N° du produit ABIN6256153

Cet anticorps anti-EIF3B Polyclonal Lapin (ABIN6256153) détecte spécifiquement EIF3B dans WB et IHC. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour EIF3B anticorps (pSer154) (ABIN6256153)

Antigène: EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF3B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-EIF3B anticorps

Épitope: pSer154

Specificité: Phospho-EIF3B(Ser154) Antibody detects endogenous levels of EIF3B.

Réactivité croisée: Humain, Souris, Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogène: A synthesized peptide derived from human EIF3B around the phosphorylation site of Ser154.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C.Stable for 12 months from date of receipt

Date de péremption: 12 months

Détails sur EIF3B

Antigène: EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B))

Autre désignation: EIF3B

Sujet:

Description: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:9388245, PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:9388245, PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

Gene: EIF3B

Poids moléculaire: 116 kDa

ID gène: 8662

UniProt: P55884

Pathways: Ribonucleoprotein Complex Subunit Organization