ATG14 anticorps (pSer29)

N° du produit ABIN6256242

L’anticorps anti-ATG14 Polyclonal Lapin est utilisé pour la détection de ATG14 dans des échantillons de Humain et Souris. Il a été validé pour WB et IHC.

Aperçu rapide pour ATG14 anticorps (pSer29) (ABIN6256242)

Antigène: ATG14 (ATG14 Autophagy Related 14 (ATG14))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG14 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-ATG14 anticorps

Épitope: pSer29

Specificité: Phospho-Atg14 (Ser29) Antibody detects endogenous levels of Atg14.

Réactivité croisée: Humain, Souris

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogène: A synthesized peptide derived from human Atg14 around the phosphorylation site of Ser29.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C.Stable for 12 months from date of receipt

Date de péremption: 12 months

Détails sur ATG14

Antigène: ATG14 (ATG14 Autophagy Related 14 (ATG14))

Autre désignation: Atg14

Sujet:

Description: Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed:18843052, PubMed:19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed:20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed:23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed:25686604). Modulates the hepatic lipid metabolism (By similarity).

Gene: ATG14

Poids moléculaire: 65kDa

ID gène: 22863

UniProt: Q6ZNE5

Pathways: Autophagy