GJB2 anticorps (Cytoplasmic Domain)

N° du produit ABIN626152

Cet anticorps Souris Monoclonal détecte spécifiquement GJB2 dans WB, ELISA, IHC, IHC (p) et IHC (fro). Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour GJB2 anticorps (Cytoplasmic Domain) (ABIN626152)

Antigène: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GJB2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: CX-12H10

Détail du produit anti-GJB2 anticorps

Épitope: Cytoplasmic Domain

Marque: IHC-plus™

Specificité: Reacts strongly with Connexin-26, which has a predicted molecular weight of 26.5kD. Although the polyclonal anti-Cx26 antibody (feline. No. 71-0500) raised against the same sequence as this monoclonal anti-Cx26 antibody cross-reacts with Cx30, this monoclonal anti-Cx26 appears to exhibit minimal cross-reaction with Cx30 by western blotting. The degree of cross-reaction with Cx30 by IHC is uncertain, but may be influenced by fixation conditions. Rat, mouse and human. Reactivity of this antibody on Western blots has been confirmed by using extracts from mouse liver, mouse brain and rat brain.

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-26. (4,5).

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-Fr, IHC-P (10 μg/mL), WB (1 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 μg/mL. Immunohistochemistry (Frozen): 1-2 μg/mL. Western Blot: 1 μg/mL. Reactivity confirmed using extracts from mouse liver, mouse brain and rat brain.

Commentaires:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Aliquot to Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Détails sur GJB2

Antigène: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Autre désignation: GJB2 / CX26 / Connexin 26

Sujet: Name/Gene ID: GJB2
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJB2, Connexin 26, Connexin-26, DFNB1A, DFNA3, DFNB1, Gap junction protein beta 2, KID, HID, PPK, NSRD1, CX26, DFNA3A

ID gène: 2706

UniProt: P29033

Pathways: Sensory Perception of Sound, Cell-Cell Junction Organization