GLI3 anticorps (Internal Region)

N° du produit ABIN6261965

Cet anticorps Lapin Polyclonal détecte spécifiquement GLI3 dans WB, IHC et ELISA. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour GLI3 anticorps (Internal Region) (ABIN6261965)

Antigène: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLI3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-GLI3 anticorps

Épitope: Internal Region

Specificité: Gli3 Antibody detects endogenous levels of total Gli3.

Homologie: Pig,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human Gli3, corresponding to a region within the internal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur GLI3

Antigène: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Autre désignation: GLI3

Sujet:

Description: Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.

Gene: GLI3

Poids moléculaire: 170 kDa

ID gène: 2737

UniProt: P10071

Pathways: Signalisation Hedgehog